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Lexogen GmbH poly(a) rna selection kit v1.5
Poly(a) Rna Selection Kit V1.5, supplied by Lexogen GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/poly+(a)+rna+selection+kit/poly+a++rna+selection+kit/pmc12276004-129-13-18
Average 90 stars, based on 1 article reviews
poly(a) rna selection kit v1.5 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

RNA Sequencing:

Article Title: Methylome analysis of FTLD patients with TDP-43 pathology identifies epigenetic signatures specific to pathological subtypes.
Article Snippet: Total RNAs extracted from iPSC-derived neurons were then subjected to a polyA enrichment step, using the Poly(A) RNA Selection Kit (V1.5, Lexogen, Vienna, Austria). cDNA libraries were prepared using the direct cDNA sequencing Kit with native barcoding (Oxford Nanopore Technologies, Oxford, UK) and sequenced on a PromethION24 sequencer (Oxford Nanopore Technologies, Oxford, UK).

Article Title: Extracellular matrix glycation epigenetically regulates brain aging and neurodegeneration in the in vitro aged neurovascular model
Article Snippet: Libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA-Seq Kit (New England BioLabs, Ipswich, MA, USA). mRNA isolation was performed using a Poly(A) RNA Selection Kit (LEXOGEN, Vienna, Austria).

Article Title: BAHCC1 promotes gene expression in neuronal cells by antagonizing SIN3A–HDAC1
Article Snippet: Prior to library generation, 1 μg of total RNA was PolyA-enriched using the Poly(A) RNA Selection Kit V1.5 (Lexogen).

Article Title: Targeted inhibition of Ninjurin2 promotes chemosensitivity in chemoresistant gastric cancer by suppressing cancer-initiating cells.
Article Snippet: The mRNA was isolated from the total RNA using a Poly(A) RNA selection kit (LEXOGEN, Austria), and cDNA was synthesized following the manufacturer’s protocol.

Article Title: Analysis of the splicing landscape of the frontal cortex in FTLD-TDP reveals subtype specific patterns and cryptic splicing
Article Snippet: Following RNA extraction, a polyA enrichment step was performed using the Poly(A) RNA Selection Kit (V1.5, Lexogen).

Article Title: Discovery and genomic characterization of Ulleung virus harbored by Crocidura utsuryoensis on Ulleung Island in Republic of Korea
Article Snippet: Library preparation was performed using the NEBNext Ultra II directional RNA-sequencing kit (New England Biolabs, Ipswich, MA, USA). mRNA (without rRNA and tRNA) was isolated using the poly(A) RNA selection kit (Lexogen, Vienna, Austria). cDNA was synthesized from the isolated mRNA and subsequently fragmented according to the manufacturer’s guidelines.

Article Title: Chromosome-level genome of Zoysia sinica in the intertidal zone reveals genomic insights into waterlogging stress adaptation.
Article Snippet: For RNA sequencing, mRNA was enriched using the Poly(A) RNA Selection Kit (Lexogen).

Article Title: Genetic association of intelligence with longevity in Drosophila melanogaster.
Article Snippet: The isolation of mRNA was performed using Poly (A) RNA Selection Kit (LEXOGEN, Inc., Austria).

Selection:

Article Title: Methylome analysis of FTLD patients with TDP-43 pathology identifies epigenetic signatures specific to pathological subtypes.
Article Snippet: Total RNAs extracted from iPSC-derived neurons were then subjected to a polyA enrichment step, using the Poly(A) RNA Selection Kit (V1.5, Lexogen, Vienna, Austria). cDNA libraries were prepared using the direct cDNA sequencing Kit with native barcoding (Oxford Nanopore Technologies, Oxford, UK) and sequenced on a PromethION24 sequencer (Oxford Nanopore Technologies, Oxford, UK).

Article Title: Extracellular matrix glycation epigenetically regulates brain aging and neurodegeneration in the in vitro aged neurovascular model
Article Snippet: Libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA-Seq Kit (New England BioLabs, Ipswich, MA, USA). mRNA isolation was performed using a Poly(A) RNA Selection Kit (LEXOGEN, Vienna, Austria).

Article Title: BAHCC1 promotes gene expression in neuronal cells by antagonizing SIN3A–HDAC1
Article Snippet: Prior to library generation, 1 μg of total RNA was PolyA-enriched using the Poly(A) RNA Selection Kit V1.5 (Lexogen).

Article Title: Targeted inhibition of Ninjurin2 promotes chemosensitivity in chemoresistant gastric cancer by suppressing cancer-initiating cells.
Article Snippet: The mRNA was isolated from the total RNA using a Poly(A) RNA selection kit (LEXOGEN, Austria), and cDNA was synthesized following the manufacturer’s protocol.

Article Title: Analysis of the splicing landscape of the frontal cortex in FTLD-TDP reveals subtype specific patterns and cryptic splicing
Article Snippet: Following RNA extraction, a polyA enrichment step was performed using the Poly(A) RNA Selection Kit (V1.5, Lexogen).

Article Title: Discovery and genomic characterization of Ulleung virus harbored by Crocidura utsuryoensis on Ulleung Island in Republic of Korea
Article Snippet: Library preparation was performed using the NEBNext Ultra II directional RNA-sequencing kit (New England Biolabs, Ipswich, MA, USA). mRNA (without rRNA and tRNA) was isolated using the poly(A) RNA selection kit (Lexogen, Vienna, Austria). cDNA was synthesized from the isolated mRNA and subsequently fragmented according to the manufacturer’s guidelines.

Article Title: Chromosome-level genome of Zoysia sinica in the intertidal zone reveals genomic insights into waterlogging stress adaptation.
Article Snippet: For RNA sequencing, mRNA was enriched using the Poly(A) RNA Selection Kit (Lexogen).

Article Title: Genetic association of intelligence with longevity in Drosophila melanogaster.
Article Snippet: The isolation of mRNA was performed using Poly (A) RNA Selection Kit (LEXOGEN, Inc., Austria).

Sequencing:

Article Title: Methylome analysis of FTLD patients with TDP-43 pathology identifies epigenetic signatures specific to pathological subtypes.
Article Snippet: Total RNAs extracted from iPSC-derived neurons were then subjected to a polyA enrichment step, using the Poly(A) RNA Selection Kit (V1.5, Lexogen, Vienna, Austria). cDNA libraries were prepared using the direct cDNA sequencing Kit with native barcoding (Oxford Nanopore Technologies, Oxford, UK) and sequenced on a PromethION24 sequencer (Oxford Nanopore Technologies, Oxford, UK).

Article Title: Extracellular matrix glycation epigenetically regulates brain aging and neurodegeneration in the in vitro aged neurovascular model
Article Snippet: Libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA-Seq Kit (New England BioLabs, Ipswich, MA, USA). mRNA isolation was performed using a Poly(A) RNA Selection Kit (LEXOGEN, Vienna, Austria).

Article Title: BAHCC1 promotes gene expression in neuronal cells by antagonizing SIN3A–HDAC1
Article Snippet: Prior to library generation, 1 μg of total RNA was PolyA-enriched using the Poly(A) RNA Selection Kit V1.5 (Lexogen).

Article Title: Targeted inhibition of Ninjurin2 promotes chemosensitivity in chemoresistant gastric cancer by suppressing cancer-initiating cells.
Article Snippet: The mRNA was isolated from the total RNA using a Poly(A) RNA selection kit (LEXOGEN, Austria), and cDNA was synthesized following the manufacturer’s protocol.

Article Title: Analysis of the splicing landscape of the frontal cortex in FTLD-TDP reveals subtype specific patterns and cryptic splicing
Article Snippet: Following RNA extraction, a polyA enrichment step was performed using the Poly(A) RNA Selection Kit (V1.5, Lexogen).

Article Title: Discovery and genomic characterization of Ulleung virus harbored by Crocidura utsuryoensis on Ulleung Island in Republic of Korea
Article Snippet: Library preparation was performed using the NEBNext Ultra II directional RNA-sequencing kit (New England Biolabs, Ipswich, MA, USA). mRNA (without rRNA and tRNA) was isolated using the poly(A) RNA selection kit (Lexogen, Vienna, Austria). cDNA was synthesized from the isolated mRNA and subsequently fragmented according to the manufacturer’s guidelines.

Article Title: Chromosome-level genome of Zoysia sinica in the intertidal zone reveals genomic insights into waterlogging stress adaptation.
Article Snippet: For RNA sequencing, mRNA was enriched using the Poly(A) RNA Selection Kit (Lexogen).

Article Title: Genetic association of intelligence with longevity in Drosophila melanogaster.
Article Snippet: The isolation of mRNA was performed using Poly (A) RNA Selection Kit (LEXOGEN, Inc., Austria).

Isolation:

Article Title: Methylome analysis of FTLD patients with TDP-43 pathology identifies epigenetic signatures specific to pathological subtypes.
Article Snippet: Total RNAs extracted from iPSC-derived neurons were then subjected to a polyA enrichment step, using the Poly(A) RNA Selection Kit (V1.5, Lexogen, Vienna, Austria). cDNA libraries were prepared using the direct cDNA sequencing Kit with native barcoding (Oxford Nanopore Technologies, Oxford, UK) and sequenced on a PromethION24 sequencer (Oxford Nanopore Technologies, Oxford, UK).

Article Title: Extracellular matrix glycation epigenetically regulates brain aging and neurodegeneration in the in vitro aged neurovascular model
Article Snippet: Libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA-Seq Kit (New England BioLabs, Ipswich, MA, USA). mRNA isolation was performed using a Poly(A) RNA Selection Kit (LEXOGEN, Vienna, Austria).

Article Title: BAHCC1 promotes gene expression in neuronal cells by antagonizing SIN3A–HDAC1
Article Snippet: Prior to library generation, 1 μg of total RNA was PolyA-enriched using the Poly(A) RNA Selection Kit V1.5 (Lexogen).

Article Title: Targeted inhibition of Ninjurin2 promotes chemosensitivity in chemoresistant gastric cancer by suppressing cancer-initiating cells.
Article Snippet: The mRNA was isolated from the total RNA using a Poly(A) RNA selection kit (LEXOGEN, Austria), and cDNA was synthesized following the manufacturer’s protocol.

Article Title: Analysis of the splicing landscape of the frontal cortex in FTLD-TDP reveals subtype specific patterns and cryptic splicing
Article Snippet: Following RNA extraction, a polyA enrichment step was performed using the Poly(A) RNA Selection Kit (V1.5, Lexogen).

Article Title: Discovery and genomic characterization of Ulleung virus harbored by Crocidura utsuryoensis on Ulleung Island in Republic of Korea
Article Snippet: Library preparation was performed using the NEBNext Ultra II directional RNA-sequencing kit (New England Biolabs, Ipswich, MA, USA). mRNA (without rRNA and tRNA) was isolated using the poly(A) RNA selection kit (Lexogen, Vienna, Austria). cDNA was synthesized from the isolated mRNA and subsequently fragmented according to the manufacturer’s guidelines.

Article Title: Chromosome-level genome of Zoysia sinica in the intertidal zone reveals genomic insights into waterlogging stress adaptation.
Article Snippet: For RNA sequencing, mRNA was enriched using the Poly(A) RNA Selection Kit (Lexogen).

Article Title: Genetic association of intelligence with longevity in Drosophila melanogaster.
Article Snippet: The isolation of mRNA was performed using Poly (A) RNA Selection Kit (LEXOGEN, Inc., Austria).

Synthesized:

Article Title: Methylome analysis of FTLD patients with TDP-43 pathology identifies epigenetic signatures specific to pathological subtypes.
Article Snippet: Total RNAs extracted from iPSC-derived neurons were then subjected to a polyA enrichment step, using the Poly(A) RNA Selection Kit (V1.5, Lexogen, Vienna, Austria). cDNA libraries were prepared using the direct cDNA sequencing Kit with native barcoding (Oxford Nanopore Technologies, Oxford, UK) and sequenced on a PromethION24 sequencer (Oxford Nanopore Technologies, Oxford, UK).

Article Title: Extracellular matrix glycation epigenetically regulates brain aging and neurodegeneration in the in vitro aged neurovascular model
Article Snippet: Libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA-Seq Kit (New England BioLabs, Ipswich, MA, USA). mRNA isolation was performed using a Poly(A) RNA Selection Kit (LEXOGEN, Vienna, Austria).

Article Title: BAHCC1 promotes gene expression in neuronal cells by antagonizing SIN3A–HDAC1
Article Snippet: Prior to library generation, 1 μg of total RNA was PolyA-enriched using the Poly(A) RNA Selection Kit V1.5 (Lexogen).

Article Title: Targeted inhibition of Ninjurin2 promotes chemosensitivity in chemoresistant gastric cancer by suppressing cancer-initiating cells.
Article Snippet: The mRNA was isolated from the total RNA using a Poly(A) RNA selection kit (LEXOGEN, Austria), and cDNA was synthesized following the manufacturer’s protocol.

Article Title: Analysis of the splicing landscape of the frontal cortex in FTLD-TDP reveals subtype specific patterns and cryptic splicing
Article Snippet: Following RNA extraction, a polyA enrichment step was performed using the Poly(A) RNA Selection Kit (V1.5, Lexogen).

Article Title: Discovery and genomic characterization of Ulleung virus harbored by Crocidura utsuryoensis on Ulleung Island in Republic of Korea
Article Snippet: Library preparation was performed using the NEBNext Ultra II directional RNA-sequencing kit (New England Biolabs, Ipswich, MA, USA). mRNA (without rRNA and tRNA) was isolated using the poly(A) RNA selection kit (Lexogen, Vienna, Austria). cDNA was synthesized from the isolated mRNA and subsequently fragmented according to the manufacturer’s guidelines.

Article Title: Chromosome-level genome of Zoysia sinica in the intertidal zone reveals genomic insights into waterlogging stress adaptation.
Article Snippet: For RNA sequencing, mRNA was enriched using the Poly(A) RNA Selection Kit (Lexogen).

Article Title: Genetic association of intelligence with longevity in Drosophila melanogaster.
Article Snippet: The isolation of mRNA was performed using Poly (A) RNA Selection Kit (LEXOGEN, Inc., Austria).

RNA Extraction:

Article Title: Methylome analysis of FTLD patients with TDP-43 pathology identifies epigenetic signatures specific to pathological subtypes.
Article Snippet: Total RNAs extracted from iPSC-derived neurons were then subjected to a polyA enrichment step, using the Poly(A) RNA Selection Kit (V1.5, Lexogen, Vienna, Austria). cDNA libraries were prepared using the direct cDNA sequencing Kit with native barcoding (Oxford Nanopore Technologies, Oxford, UK) and sequenced on a PromethION24 sequencer (Oxford Nanopore Technologies, Oxford, UK).

Article Title: Extracellular matrix glycation epigenetically regulates brain aging and neurodegeneration in the in vitro aged neurovascular model
Article Snippet: Libraries were prepared from total RNA using the NEBNext Ultra II Directional RNA-Seq Kit (New England BioLabs, Ipswich, MA, USA). mRNA isolation was performed using a Poly(A) RNA Selection Kit (LEXOGEN, Vienna, Austria).

Article Title: BAHCC1 promotes gene expression in neuronal cells by antagonizing SIN3A–HDAC1
Article Snippet: Prior to library generation, 1 μg of total RNA was PolyA-enriched using the Poly(A) RNA Selection Kit V1.5 (Lexogen).

Article Title: Targeted inhibition of Ninjurin2 promotes chemosensitivity in chemoresistant gastric cancer by suppressing cancer-initiating cells.
Article Snippet: The mRNA was isolated from the total RNA using a Poly(A) RNA selection kit (LEXOGEN, Austria), and cDNA was synthesized following the manufacturer’s protocol.

Article Title: Analysis of the splicing landscape of the frontal cortex in FTLD-TDP reveals subtype specific patterns and cryptic splicing
Article Snippet: Following RNA extraction, a polyA enrichment step was performed using the Poly(A) RNA Selection Kit (V1.5, Lexogen).

Article Title: Discovery and genomic characterization of Ulleung virus harbored by Crocidura utsuryoensis on Ulleung Island in Republic of Korea
Article Snippet: Library preparation was performed using the NEBNext Ultra II directional RNA-sequencing kit (New England Biolabs, Ipswich, MA, USA). mRNA (without rRNA and tRNA) was isolated using the poly(A) RNA selection kit (Lexogen, Vienna, Austria). cDNA was synthesized from the isolated mRNA and subsequently fragmented according to the manufacturer’s guidelines.

Article Title: Chromosome-level genome of Zoysia sinica in the intertidal zone reveals genomic insights into waterlogging stress adaptation.
Article Snippet: For RNA sequencing, mRNA was enriched using the Poly(A) RNA Selection Kit (Lexogen).

Article Title: Genetic association of intelligence with longevity in Drosophila melanogaster.
Article Snippet: The isolation of mRNA was performed using Poly (A) RNA Selection Kit (LEXOGEN, Inc., Austria).



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A-C RT-PCR analysis of relative Piezo1 <t>mRNA</t> expression in primary microglia (A), astrocyte (B) and neuron (C) upon normoxia and OGD/R condition, with normoxia set to 1.0 ( n = 4 per group). D RT-PCR analysis of the relative Piezo1 mRNA expression in the MACS microglia isolated from mice subjected to sham operation or MCAO/R injury, with sham brain set to 1.0. E, F Western blot analysis of Piezo1 in MACS microglia isolated from mice subjected to sham operation or MCAO/R injury. A representative image was shown in E panel. Quantification of Piezo1 protein in indicated groups was shown in F panel by taking sham brain set to 1.0. G, H Representative fura-2 Ca 2+ imaging of primary cultured microglia upon Normoxia or OGD/R condition response to Yoda1 (G) and ATP (H). I Scatterplot of maximal fura-2 amplitude changes in response to Yoda1 or ATP in indicated groups. J Representative immunofluorescence images of Piezo1 with Iba1 in the peri-infarcted regions after MCAO/R. Scale bar, 50 µm. K, L Quantification of the percentage of Piezo1 + cells among Iba1 + cells in peri-infarcted regions (K), and infarct cores (L). Data are expressed as mean ± SD. One-way ANOVA with Dunnett’s post hoc test for A, B, C, K and L. Two tailed unpaired Student’s t test for I. Kruskal–Wallis test with Dunn’s post hoc test for D, F. ** P < 0.01, *** P < 0.001, n.s.: no significant.
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Image Search Results


A-C RT-PCR analysis of relative Piezo1 mRNA expression in primary microglia (A), astrocyte (B) and neuron (C) upon normoxia and OGD/R condition, with normoxia set to 1.0 ( n = 4 per group). D RT-PCR analysis of the relative Piezo1 mRNA expression in the MACS microglia isolated from mice subjected to sham operation or MCAO/R injury, with sham brain set to 1.0. E, F Western blot analysis of Piezo1 in MACS microglia isolated from mice subjected to sham operation or MCAO/R injury. A representative image was shown in E panel. Quantification of Piezo1 protein in indicated groups was shown in F panel by taking sham brain set to 1.0. G, H Representative fura-2 Ca 2+ imaging of primary cultured microglia upon Normoxia or OGD/R condition response to Yoda1 (G) and ATP (H). I Scatterplot of maximal fura-2 amplitude changes in response to Yoda1 or ATP in indicated groups. J Representative immunofluorescence images of Piezo1 with Iba1 in the peri-infarcted regions after MCAO/R. Scale bar, 50 µm. K, L Quantification of the percentage of Piezo1 + cells among Iba1 + cells in peri-infarcted regions (K), and infarct cores (L). Data are expressed as mean ± SD. One-way ANOVA with Dunnett’s post hoc test for A, B, C, K and L. Two tailed unpaired Student’s t test for I. Kruskal–Wallis test with Dunn’s post hoc test for D, F. ** P < 0.01, *** P < 0.001, n.s.: no significant.

Journal: bioRxiv

Article Title: Microglia Piezo1 aggravates cerebral ischemia and reperfusion injury by chemotactic recruitment of T lymphocytes

doi: 10.64898/2026.01.12.699156

Figure Lengend Snippet: A-C RT-PCR analysis of relative Piezo1 mRNA expression in primary microglia (A), astrocyte (B) and neuron (C) upon normoxia and OGD/R condition, with normoxia set to 1.0 ( n = 4 per group). D RT-PCR analysis of the relative Piezo1 mRNA expression in the MACS microglia isolated from mice subjected to sham operation or MCAO/R injury, with sham brain set to 1.0. E, F Western blot analysis of Piezo1 in MACS microglia isolated from mice subjected to sham operation or MCAO/R injury. A representative image was shown in E panel. Quantification of Piezo1 protein in indicated groups was shown in F panel by taking sham brain set to 1.0. G, H Representative fura-2 Ca 2+ imaging of primary cultured microglia upon Normoxia or OGD/R condition response to Yoda1 (G) and ATP (H). I Scatterplot of maximal fura-2 amplitude changes in response to Yoda1 or ATP in indicated groups. J Representative immunofluorescence images of Piezo1 with Iba1 in the peri-infarcted regions after MCAO/R. Scale bar, 50 µm. K, L Quantification of the percentage of Piezo1 + cells among Iba1 + cells in peri-infarcted regions (K), and infarct cores (L). Data are expressed as mean ± SD. One-way ANOVA with Dunnett’s post hoc test for A, B, C, K and L. Two tailed unpaired Student’s t test for I. Kruskal–Wallis test with Dunn’s post hoc test for D, F. ** P < 0.01, *** P < 0.001, n.s.: no significant.

Article Snippet: Paired-end sequencing libraries were performed using a mRNA NEBNext Poly selection Library Prep Kit (Illumina, Los Angeles, CA, USA). cDNA sequence was then conducted using the Illumina NovaSeq 6000 sequencing platform with multiplex paired-end 150 base pairs reads.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Isolation, Western Blot, Imaging, Cell Culture, Immunofluorescence, Two Tailed Test

A,B Heatmap plot of multiplexed immunoassay showing chemokine expression profiles in the ischemic brains of indicated groups. Quantification of chemokine concentrations in indicated groups is shown in panel B, results were generated according to clustering of proteome profiler array assays of the listed chemokine. C Cxcl10 mRNA levels detected by RT-PCR in peri-infarct area of Piezo1 CKO mice within the first 72 h after MCAO/R. D.E Western blot analysis of CXCL10 in peri-infarct area separated from control and Piezo1 CKO mice subjected to sham operation or MCAO/R injury. Reprehensive image was shown in panel D. Quantification of CXCL10 protein in indicated groups was shown in panel E, with sham brain set to 1.0. F Immunostaining for CXCL10 and GFAP of brain sections 72 h after MCAO/R. Scale bar, 50 µm. G Quantitative analysis of the GFAP and CXCL10 positive cells number in panel F. H Representative Evans blue staining images of brain sections. I Quantitative analysis of the Evans blue staining volume in panel H. Each symbol represents one mouse. Data are expressed as mean ± SD. Two-tailed unpaired t-test for B and G. Two-way ANOVA followed by Sidak’s post hoc test for C and E. One-way ANOVA followed by Dunnett’s post hoc test for I. * P < 0.05, ** P < 0.01, *** P < 0.001, n.s.: no significant.

Journal: bioRxiv

Article Title: Microglia Piezo1 aggravates cerebral ischemia and reperfusion injury by chemotactic recruitment of T lymphocytes

doi: 10.64898/2026.01.12.699156

Figure Lengend Snippet: A,B Heatmap plot of multiplexed immunoassay showing chemokine expression profiles in the ischemic brains of indicated groups. Quantification of chemokine concentrations in indicated groups is shown in panel B, results were generated according to clustering of proteome profiler array assays of the listed chemokine. C Cxcl10 mRNA levels detected by RT-PCR in peri-infarct area of Piezo1 CKO mice within the first 72 h after MCAO/R. D.E Western blot analysis of CXCL10 in peri-infarct area separated from control and Piezo1 CKO mice subjected to sham operation or MCAO/R injury. Reprehensive image was shown in panel D. Quantification of CXCL10 protein in indicated groups was shown in panel E, with sham brain set to 1.0. F Immunostaining for CXCL10 and GFAP of brain sections 72 h after MCAO/R. Scale bar, 50 µm. G Quantitative analysis of the GFAP and CXCL10 positive cells number in panel F. H Representative Evans blue staining images of brain sections. I Quantitative analysis of the Evans blue staining volume in panel H. Each symbol represents one mouse. Data are expressed as mean ± SD. Two-tailed unpaired t-test for B and G. Two-way ANOVA followed by Sidak’s post hoc test for C and E. One-way ANOVA followed by Dunnett’s post hoc test for I. * P < 0.05, ** P < 0.01, *** P < 0.001, n.s.: no significant.

Article Snippet: Paired-end sequencing libraries were performed using a mRNA NEBNext Poly selection Library Prep Kit (Illumina, Los Angeles, CA, USA). cDNA sequence was then conducted using the Illumina NovaSeq 6000 sequencing platform with multiplex paired-end 150 base pairs reads.

Techniques: Expressing, Generated, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Immunostaining, Staining, Two Tailed Test

A Relative cytokine mRNA levels in the peri-infarct area of control and Piezo1 CKO mice were detected by RT-PCR analysis 72 h after MCAO/R. B TNF-α in in peri-infarct area of control and Piezo1 CKO mice were detected by western blot analysis 72 h after MCAO/R. C Quantification of TNF-α of indicated groups, normalized to controls (set as 1.0). D flow cytometry analysis of IFN-γ positive cells in peri-infarct area of control and Piezo1 CKO mice 72 h after MCAO/R. E Quantitative analysis of IFN-γ positive cells in panel D. F, G Tnf-α (F) and Ifn-γ (G) mRNA level in peri-infarct area of control and Piezo1 CKO mice within the first 72 h after MCAO/R. H Flow cytometry analysis of TNF-α positive cells in MACS microglia isolated from peri-infarct area of control and Piezo1 CKO mice 72 h after MCAO/R. I Quantitative analysis of TNF-α positive cells in panel H. H Flow cytometry analysis of IFN-γ positive cells in MACS microglia isolated from peri-infarct area of control and Piezo1 CKO mice 72 h after MCAO/R. I Quantitative analysis of IFN-γ positive cells in panel H. L Volcano plot analysis showing dysregulated genes related to IFN-γ signaling performed in MACS microglia isolated from control and Piezo1 CKO mice at 72 h after MCAO/R. M Pathway enrichment analysis using Reactome datasets displaying the top 20 terms compared between control and Piezo1 CKO mice at 72 h after MCAO/R. N Flow cytometry analysis of CXCL10 positive astrocytes in astrocytes and control of Piezo1 CKO microglia co-culture system treated by TNF-α and/or IFN-γ antibodies upon OGD/R condition. O Quantitative analysis of CXCL10 positive cells in panel N. Data are expressed as mean ± SD. Two-tailed unpaired t-test for A, C, E, I and K. Two-way ANOVA with Sidak’s post hoc test in F, G. One-way ANOVA followed by Dunnett’s post hoc test for O. * P < 0.05, ** P < 0.01, *** P < 0.001, n.s.: no significant.

Journal: bioRxiv

Article Title: Microglia Piezo1 aggravates cerebral ischemia and reperfusion injury by chemotactic recruitment of T lymphocytes

doi: 10.64898/2026.01.12.699156

Figure Lengend Snippet: A Relative cytokine mRNA levels in the peri-infarct area of control and Piezo1 CKO mice were detected by RT-PCR analysis 72 h after MCAO/R. B TNF-α in in peri-infarct area of control and Piezo1 CKO mice were detected by western blot analysis 72 h after MCAO/R. C Quantification of TNF-α of indicated groups, normalized to controls (set as 1.0). D flow cytometry analysis of IFN-γ positive cells in peri-infarct area of control and Piezo1 CKO mice 72 h after MCAO/R. E Quantitative analysis of IFN-γ positive cells in panel D. F, G Tnf-α (F) and Ifn-γ (G) mRNA level in peri-infarct area of control and Piezo1 CKO mice within the first 72 h after MCAO/R. H Flow cytometry analysis of TNF-α positive cells in MACS microglia isolated from peri-infarct area of control and Piezo1 CKO mice 72 h after MCAO/R. I Quantitative analysis of TNF-α positive cells in panel H. H Flow cytometry analysis of IFN-γ positive cells in MACS microglia isolated from peri-infarct area of control and Piezo1 CKO mice 72 h after MCAO/R. I Quantitative analysis of IFN-γ positive cells in panel H. L Volcano plot analysis showing dysregulated genes related to IFN-γ signaling performed in MACS microglia isolated from control and Piezo1 CKO mice at 72 h after MCAO/R. M Pathway enrichment analysis using Reactome datasets displaying the top 20 terms compared between control and Piezo1 CKO mice at 72 h after MCAO/R. N Flow cytometry analysis of CXCL10 positive astrocytes in astrocytes and control of Piezo1 CKO microglia co-culture system treated by TNF-α and/or IFN-γ antibodies upon OGD/R condition. O Quantitative analysis of CXCL10 positive cells in panel N. Data are expressed as mean ± SD. Two-tailed unpaired t-test for A, C, E, I and K. Two-way ANOVA with Sidak’s post hoc test in F, G. One-way ANOVA followed by Dunnett’s post hoc test for O. * P < 0.05, ** P < 0.01, *** P < 0.001, n.s.: no significant.

Article Snippet: Paired-end sequencing libraries were performed using a mRNA NEBNext Poly selection Library Prep Kit (Illumina, Los Angeles, CA, USA). cDNA sequence was then conducted using the Illumina NovaSeq 6000 sequencing platform with multiplex paired-end 150 base pairs reads.

Techniques: Control, Reverse Transcription Polymerase Chain Reaction, Western Blot, Flow Cytometry, Isolation, Co-Culture Assay, Two Tailed Test